Increased Subcellular Hyaluronidase in the Rat after Hydrocortisone Injection

Abstract

The activity of hyaluronidase per milligram protein was found to be significantly greater in subcellular fractions from kidneys of rats injected 12 or 18 h previously with 10 mg hydrocortisone than it was in control rats. The difference was greater at 12 h than at 6 or 18 h, and at 12 h those fractions containing lysosomes showed the greatest increase. A higher specific activity of hyaluronidase in some subcellular fractions from liver and spleen was also found after hydrocortisone. Cycloheximide, given 5 h after hydrocortisone, abolished the increase in hyaluronidase measured at 12 h. However, actinomycin D given [Formula: see text] before hydrocortisone caused only a slight decrease in the effect on hyaluronidase in kidney homogenates.