Use of double-ditelosomic and normal chromosome 1D recombinant substitution lines to map Sr33 on chromosome arm 1DS in wheat

Abstract

Chromosome 1D homozygous recombinant substitution lines derived from Triticum aestivum 'Chinese Spring' (cross 1) or 'Chinese Spring' double-ditelosomic 1D (cross 2) hybridized with a disomic substitution line of Triticum tauschii chromosome 1D in 'Chinese Spring' were used to investigate the linkage relationships among Glu-D1, encoding subunits of high molecular weight glutenin storage proteins; Gli-D1, encoding gliadin storage proteins; Sr33, conferring stem rust resistance; and the centromere. Based on analysis of 88 and 91 recombinant substitution lines of crosses 1 and 2, respectively, Sr33 is tightly linked to Gli-D1 on chromosome arm 1DS (5.6 and 7.6% recombination) and less tightly to the centromere (29.6%, cross 2) and to Glu-D1 (40.9 and 39.5%). The order of the loci is Glu-D1 – centromere – Sr33 – Gli-D1.Key words: stem rust resistance, seed storage protein, glutenin, gliadin, Triticum aestivum, Triticum tauschii.