Insertional polymorphism of a non-LTR mobile element (NLRCth1) in European populations ofChironomus riparius(Diptera, Chironomidae) as detected by transposon insertion display

Abstract

The midge Chironomus riparius is distributed all over the Palearctic region and is well characterized both at the morphological and cytogenetic levels. Here we describe a population study based on the insertional polymorphism of the retroposon NLRCth1, by means of a S-SAP (sequence-specific amplification polymorphism) derived technique (transposon insertion display; TID). While a previous study of allozyme polymorphism in Russian samples showed little variability, all the amplicons we identified are polymorphic. Genetic distances between 6 natural populations were calculated according to Nei and did not show a positive correlation with geographic distances. The genetic diversity detected among individuals of a given population was one order of magnitude higher than that among populations. However, the value of ΦSTwas significant (p < 0.001) and indicates that natural populations are more genetically differentiated than random samples of individuals.Key words: retrotransposable elements, genetic differentiation, Chironomus riparius, sequence-specific amplification polymorphism.