Cellulase complex of a mesophilic Streptomyces strain

Author:

Ishaque M.,Kluepfel D.

Abstract

Various cellulolytic streptomycetes were isolated from natural environments. The cellulase production of one of these mesophilic Streptomyces strains, identified as S. flavogriseus was studied. When grown as a submerged culture with 1% microcrystalline cellulose (Avicel) as substrate, the strain produced considerable amounts of β-1, 4-glucan glucanohydrolase (EC 3.2.1.4; CM cellulase) in the extracellular supernatant and exhibited good overall cellulolytic activity as measured using filter paper (FP cellulase) and cotton as substrates. The maximum enzyme yields were obtained after 72 h of incubation at 30 °C. The optimal conditions for the FP cellulase activity were found at pH 5.6 and 40 °C. All β-glucosidase and cellobiase activities were found in the mycelial fraction of the culture and could be obtained in soluble form by sonication.

Publisher

Canadian Science Publishing

Subject

Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology

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