RELATIONSHIP BETWEEN NUCLEIC ACIDS, HISTONE AND NON-HISTONE PROTEIN SYNTHESIS IN HUMAN LYMPHOCYTES STIMULATED WITH PHYTOHEMAGGLUTININ

Abstract

The temporal relationship between the synthesis of histone and non-histone proteins and nucleic acids has been investigated by autoradiography in PHA stimulated human lymphocytes throughout the 72-hour culture period. It was found that a significant amount of3H-arginine and3H-lysine incorporation took place at a time when the cells were actively synthesizing DNA. Non-histone protein synthesis, as evidenced by3H-tryptophan incorporation, was found to be dependent on PHA stimulation in human lymphocytes. Inhibition of histone and/or non-histone protein synthesis leads to the inhibition of nucleic acid synthesis.