C-banding polymorphisms in several accessions of Triticum tauschii (Aegilops squarrosa)

Abstract

A generalized C-banded karyotype of Triticum tauschii (Aegilops squarrosa) was established based on chromosome analysis of 15 accessions of diverse origin, including the two varieties eusquarrosa (with the former varieties typica, anathera, and meyeri) and strangulata. The C-banding pattern of T. tauschii chromosomes was similar to the D-genome chromosomes of cultivated bread wheat, T. aestivum, thus permitting their unequivocal identification and homoeologous chromosome designations. Whereas only minor variation in C-banding pattern was observed within the accessions, a large amount of polymorphic variation was found among the different accessions. However, this polymorphic variation did not prevent chromosome identification in these lines. One accession (TA 2462) was found to be homozygous for a reciprocal translocation involving the complete arms of chromosomes 1D and 7D. In situ hybridization using the D-genome specific probe pAS1 confirmed the presence of T1DS-7DL and T7DS.1DL translocation in the accession TA 2462.Key words: Triticum tauschii, C-banding, in situ hybridization.