Microcarrier culture of fish cells and viruses in cell culture bioreactor

Abstract

This article deals with the culture of grass carp (Ctenopharyngodon idellus) lip and embryo cells on Cytodex 3 and GT-2 microcarriers in a 1.5-L cell culture bioreactor to propagate grass carp hemorrhage virus. The cells and viruses were successfully cultivated at 26 °C, pH 7.0, and dissolved oxygen 40% of air saturation. The cell density achievedwas as high as 7.4 × 106 cells/mL, and the virus titre reached 6.75 log LD50/0.5 mL from an initial 3.00 log LD50/0.5 mL. The results present broad prospects for fish virus vaccine production. Key words: fish cell, hemorrhage virus, microcarrier, cell culture.