Chromosomal location of adenylate kinase, 6-phosphogluconate dehydrogenase, and glutamate-pyruvate transaminase structural loci in wheat, barley, and Lophopyrum elongatum

Abstract

The chromosome locations of loci encoding isozymes of adenylate kinase, EC 2.7.4.3 (ADK), 6-phosphogluconate dehydrogenase, EC 1.1.1.44 (PGD), and glutamate–pyruvate transaminase, EC 2.6.1.2 (GPT) were investigated in wheat (Triticum aestivum L.), Lophopyrum elongatum (Host) Löve, and barley (Hordeum vulgare L.). Loci encoding ADK-1 are on homoeologous chromosome arms 7AL, 7BL, and 7DL in wheat, chromosome arm 7Eβ in L. elongatum, and chromosome arm 7HS in barley. The loci are designated Adk-A1, Adk-B1, Adk-D1, Adk-E1, and Adk-H1, respectively. The Adk-D1 locus is proximal in the linkage group of chromosome arm 7DL and is 24 cM from proximal locus Rc3 on the chromosome arm 7DS. A second locus, Adk-2, is on chromosome arm 6HL in barley. Locus Pgd-E2 in L. elongatum is on the long arm of chromosome 1E. This assignment agrees with the location of the barley Pgd-2 locus on the long arm of barley chromosome 1H. An attempt to assign loci encoding PGD-2 to wheat chromosomes was unsuccessful, presumably owing to gene triplication. Isozyme GPT-1 is encoded by structural genes on L. elongatum chromosome arm 1ES and tentatively on wheat chromosome arms 1AS, 1BS, and 1DS. The loci are designated Gpt-A1, Gpt-B1, Gpt-D1, and Gpt-E1, respectively. Circumstantial evidence suggests that GPT-1 is also encoded by barley chromosome 1H.Key words: Triticum, Agropyron, Hordeum, linkage.