The Proteome Response of Salt-Sensitive Rapeseed (Brassica napus L.) Genotype to Salt Stress

Author:

DOLATABADI Nima,TOORCHI Mahmoud,VALIZADEH Mostafa,BANDEHAGH Ali

Abstract

Productivity of rapeseed (Brassica napus L.), the third most important oilseed crop, was reduced more than other crops under the salt stress higher than the threshold. Thus, breeding, especially at seedling stage, seems necessary. Plants under salt stress, by synthesis of essential metabolites, specific structural proteins or enzymes of metabolic pathways deal with the stress. To identify the molecular mechanisms of salt responsiveness in rapeseed, ‘Option500’ a salt-sensitive genotype was exposed to 0, 150, and 300mM NaCl during the seedling stage. An increase in proline and the Na+ content of leaf and a reduction in shoot dry weight, plant height, K+ content and K+/Na+ ratio were observed. Protein expression changes were examined by two-dimensional electrophoresis (2-DE). Out of 110 protein spots identified by 2-DE gels, 37 spots showed significant abundant changes based on induction factor (IF), and 7 spots were recognized significantly at 5% probability level, which 1 and 6 spots were up and down-regulated, respectively. By using LC-MS/MS mass spectrometry analysis, proteins were identified which are involved in energy production and photosynthesis. Activity of enzymes involved in energy production decreased under stress, while the abundance of Phosphoribulokinase (PRK) -an important enzyme in the pentose phosphate pathway- increased.

Publisher

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Subject

Horticulture,Plant Science,Agronomy and Crop Science

Cited by 10 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Plant Proteome in Response to Abiotic Stresses;Abiotic Stress in Crop Plants - Ecophysiological Responses and Molecular Approaches;2024-07-17

2. Phenotype, Biomass, Carbon and Nitrogen Assimilation, and Antioxidant Response of Rapeseed under Salt Stress;Plants;2024-05-28

3. Alone or Combined Effect of Seaweed and Humic Acid Applications on Rapeseed (Brassica napus L.) under Salinity Stress;Journal of Soil Science and Plant Nutrition;2024-04-19

4. Role of tillage measures in mitigating waterlogging damage in rapeseed;BMC Plant Biology;2023-05-01

5. Designing the Rapeseed Genome for Abiotic Stress Tolerance;Genomic Designing for Abiotic Stress Resistant Oilseed Crops;2022

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