Radial Segmentation of Blood Lymphocytes Nuclei in Pheasants Vaccinated against Newcastle Disease and Haemorrhagic Enteritis

Abstract

The purpose of the experiment was to investigate the influence of the pheasant vaccination with a vaccine containing live attenuated virus of haemorrhagic enteritis, (HE) on the ability of peripheral blood lymphocytes to form a nuclei deformation described as the radial segmentation (RS). This feature is identified with cytoskeletal rearrangement of the cells. The pheasants were vaccinated against Newcastle disease (ND) on the 1st, 28th and 56th day of life. Moreover, on the 49th day of life, a part of the birds was given the vaccine containing HEV (Haemorrhagic enteritis virus) in drinking water. Fourteen days after the HEV vaccination, the birds were administered intravenously 0.5 ml of the 10% SRBC (Sheep red blood cells) suspension. Blood samples were collected on the 21st day after HEV vaccination, on the 7th day after SRBC injection. It was shown that in pheasants the percentage of cells undergoing RS induced by oxalates was low, approximately 3.75%, whereas in HEV vaccinated birds this percentage increased twice (p < 0.05). Pheasant immunization with SRBC led to significant reduction of the ability to form RS, whereas in HEV vaccinated birds after SRBC administration the percentage of cells with RS remained on the level of the control group. Based on these results, the authors suggest that immunosuppressive effects of some viruses may be a result of both sensitivity and rearrangement ability changes in cytoskeletal proteins of the cells involved in immunological response.