IMPACT OF ACUTE EXPERIMENTAL BLOOD LOSS ON DIURESIS AND FILTERING CAPACITY OF KIDNEYS AND EFFICACY OF CORRECTION WITH RINGER’S LACTATE SOLUTION IN COMBINATION WITH 2-ETHYL-6-METHYL-3-HYDROXYPYRIDINE SUCCINATE

Abstract

Introduction: The kidneys remain one of target organs in acute blood loss of various genesis. Reduced perfusion leads to an increase in renal insufficiency, primarily characterized by decreased urine production and glomerular filtration rate. Timely restoration of lost blood volume deficit stands as a critical objective in addressing hypovolemic shock. Among the first-line therapeutic approaches, the administration of balanced crystalloids in a 1:1 ratio relative to the lost blood volume has demonstrated efficacy; the combined use of antioxidants is regarded as promising. This study aims to investigate the impact of acute experimental blood loss on diuresis and kidney filtration capacity, assess the effectiveness of correction with Ringer's lactate solution in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate. Materials and Methods: In our experiments, we induced acute blood loss equivalent to 2% of the body mass in rats and evaluated kidney function using a water stress method after 1, 3, and 7 days. We measured diuresis, creatinine levels in blood serum and urine, and glomerular filtration rate. To address the effects of blood loss, we administered Ringer's lactate solution separately and in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate. Results: Following the induction of acute blood loss, there was a significant reduction in diuresis and glomerular filtration rate, accompanied by an increase in creatinine levels in both blood serum and urine. These impairments worsened over the course of 1 to 7 days. Comparing the efficacy of infusion therapy with Ringer's lactate solution alone and in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate after 7 days revealed a significantly more pronounced increase in diuresis, glomerular filtration rate, and a reduction in urine creatinine levels in the combined therapy group. These findings suggest the promising potential of combined therapy in preventing renal dysfunction in the context of acute blood loss. Conclusions: Acute blood loss equivalent to 2% of body mass leads to disturbances in diuresis and renal filtration capacity. The administration of Ringer's lactate solution in conjunction with 2-ethyl-6-methyl-3-hydroxypyridine succinate mitigates the effects of acute blood loss and yields a more substantial improvement compared to rats treated with Ringer's lactate solution alone.