Biological potential of a genetic engineering construction encoding the gene for the human vascular endothelium growth factor-Reference-Cited by-同舟云学术

Biological potential of a genetic engineering construction encoding the gene for the human vascular endothelium growth factor

Published:2024-05-27 Issue:2 Volume:21 Page:95-103
ISSN:2524-2350
Container-title:Proceedings of the National Academy of Sciences of Belarus, Medical series
language:
Short-container-title:Vescì Nac. akad. navuk Belarusì, Ser. med. navuk

Author:

Bogdan V. G.¹,Poleshko А. G.²,Misiukevich А. Yu.²,Smirnov А. А.²,Sukhaveyeva S. V.²,Yantsevich А. V.³

Affiliation:

1. Department of Medical Sciences of the National Academy of Sciences of Belarus

2. Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus

3. Institute of Bioorganic Chemistry of the National Academy of Sciences of Belarus

Abstract

The high biological potential of the developed genetically engineered plasmid construct pcDNATM3.1(–) VEGF165 has been established. It has been proven to induce a significant increase in the expression of the gene VEGF165 in mesenchymal multipotent stromal cells and human endothelial cells and to increase the production of the VEGF165 protein by cells. The identified temporary functional activity of the genetically engineered construct in cells and the absence of genotoxic effects minimize the likelihood of oncotransformation.The created genetically engineered vector construct pcDNATM3.1(–) VEGF165 can be used to develop a gene therapy drug model that promotes angiogenesis in vivo in ischemic tissues.

Publisher

Publishing House Belorusskaya Nauka

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