Analysis of KDM3A and DBX2 gene polymorphism for differentiation of Sus scrofa domesticus duroc pigs-Reference-Cited by-同舟云学术

Analysis of KDM3A and DBX2 gene polymorphism for differentiation of Sus scrofa domesticus duroc pigs

Published:2023-05-07 Issue:2 Volume:67 Page:119-125
ISSN:2524-2431
Container-title:Doklady of the National Academy of Sciences of Belarus
language:
Short-container-title:Dokl. Akad. nauk

Author:

Kipen V. N.¹^ORCID,Mikhailova M. E.¹^ORCID,Snytkov E. V.¹^ORCID,Sheyko R. I.¹^ORCID

Affiliation:

1. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus

Abstract

Genotyping of individuals of 7 commercial breeds of domestic pigs was carried out: Duroc, Landrace, Belarusian Large White, Belarusian Black-and-White, Belarusian meat, Pietrain and Yorkshire for the KDM3A and DBX2 genes. The high breed-specific potential of polymorphic loci g.58335217A>G (KDM3A gene) and g.75953650G>T (DBX2 gene) for differentiation of Duroc pigs of the species Sus scrofa domesticus was confirmed. A test model for differentiation of Duroc individuals by bioinformatic assessment of a total contribution of two SNPs of the KDM3A and DBX2 genes was proposed. This test model has no analogues in the world, and its estimation accuracy and specificity are 98.76 and 99.68 %, respectively. Using PCR-RFLP, a fast and simple approach has been developed to differentiate the Duroc breed based on the proposed test model.

Publisher

Publishing House Belorusskaya Nauka

Subject

General Medicine

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