Affiliation:
1. Peoples’ Friendship University of Russia (RUDN)
2. Central Research Institute of Epidemiology
3. Peoples’ Friendship University of Russia (RUDN);
Moscow City Hospital named after V.V. Vinogradov
4. Lomonosov Moscow State University
Abstract
Advances in the diagnosis and treatment of patients with infectious endocarditis are limited by the high frequency of cases with an unknown etiology and imperfection of microbiological (cultural) methods. To overcome these problems new approaches to the identification of infectious endocarditis pathogens were introduced, which allowed achieving certain positive results. However, it should be noted that despite the wide variety of diagnostic tools currently used, there is no ideal method for etiological laboratory diagnosis of infectious endocarditis. The article discusses the features and place of immunochemical, molecular biological (MALDI-TOF MS, real-time PCR, sequencing, in situ fluorescence hybridization, metagenomic methods, etc.), immunohistochemical methods, and their advantages and limitations.
Publisher
Stolichnaya Izdatelskaya Kompaniyaizdat
Subject
Pharmacology (medical),Cardiology and Cardiovascular Medicine
Reference60 articles.
1. Habib G., Lancellotti P., Erba P., et al., EURO-ENDO Investigators. Clinical presentation, aetiology and outcome of infective endocarditis. Results of the ESC-EORP EURO-ENDO (European infective endocarditis) registry: a prospective cohort study. Eur Heart J. 2019;40(39):3222-32. DOI:10.1093/eurheartj/ehz620.
2. Murdoch D., Corey G., Hoen B., et. all. Clinical presentation, etiology, and outcome of infective endocarditis in the 21st century: the International Collaboration on Endocarditis-Prospective Cohort study. Arch Intern Med. 2009;169:463-73. DOI:10.1001/archinternmed.2008.603.
3. Shevchenko Yu.L., ed. Infective endocarditis, manual. Moscow: Geotar-Media; 2012 (In Russ.)
4. Danilov A.I., Alekseeva I.V., Asner T.V., et al. Real practice of therapy of infective endocarditis in the Russian Federation: intermediate results of the MAESTRO study. Clinical Microbiology and Antimicrobial Chemotherapy. 2013;15(2); suppl 1:18-9 (In Russ.)
5. Kotova E.O., Domonova E.A., Karaulova Yu.L., et al. Infective endocarditis: Importance of molecular biology techniques in the etiological diagnosis. Ter Arkhiv. 2016;88(11):62-7 (In Russ.) DOI:10.17116/terarkh2016881162-67.
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