Affiliation:
1. Hunan Key Laboratory of Mechanism and Technology of Quantum Information
2. University of Glasgow
Abstract
The 3D location of light emitters provides essential information in many biological, chemical and physical systems. 4Pi single-molecule localization microscopy (SMLM) being one of the state-of-the-art techniques provides exceptional precision in the axial direction; however, the lateral precision degrades significantly when the emitter is displaced several wavelengths away from the mutual focal plane. Here we propose a hybrid system employing type-B 4Pi microscope and engineered point-spread function (PSF), the resultant interferometric PSF is optimized against the Cramer-Rao metric, yielding the richest information regarding the emitter’s 3D locations. It outperforms conventional 4Pi SMLM with a larger axial range and compares advantageously to the non-interferometric PSFs with doubled photon efficiency and better precision in all directions.
Funder
National Natural Science Foundation of China
The Science and Technology Innovation Program of Hunan Province