GAN-based quantitative oblique back-illumination microscopy enables computationally efficient epi-mode refractive index tomography

Abstract

Quantitative oblique back-illumination microscopy (qOBM) is a novel imaging technology that enables epi-mode 3D quantitative phase imaging and refractive index (RI) tomography of thick scattering samples. The technology uses four oblique back illumination images captured at the same focal plane and a fast 2D deconvolution reconstruction algorithm to reconstruct 2D phase cross-sections of thick samples. Alternatively, a through-focus z-stack of oblique back illumination images can be used to recover 3D RI tomograms with improved RI quantitative fidelity at the cost of a more computationally expensive reconstruction algorithm. Here, we report on a generative adversarial network (GAN) assisted approach to reconstruct 3D RI tomograms with qOBM that achieves high fidelity and greatly reduces processing time. The proposed approach achieves high-fidelity 3D RI tomography using differential phase contrast images from three adjacent z-planes. A ∼9-fold improvement in volumetric reconstruction time is achieved. We further show that this technique provides high SNR RI tomograms with high quantitative fidelity, reduces motion artifacts, and generalizes to different tissue types. This work can lead to real-time, high-fidelity RI tomographic imaging for in-vivo pre-clinical and clinical applications.