Affiliation:
1. Key Laboratory of Opto-Electronics Information Technology (Tianjin University),
2. Institute of Optical Fiber Sensing of Tianjin University
3. University of Warwick
4. Tianjin University of Technology and Education
5. Shenzhen Nuoan Technology Co
Abstract
Deuterium-based isotopic labeling is an important technique for tracking cellular metabolism with the Raman signals analysis of low-wavenumber (LW) C–D bonds and high-wavenumber (HW) C–H bonds. We propose and demonstrate a disposable ultra-miniature fiber probe to detect LW and HW coherent anti-Stokes Raman scattering (CARS) spectra for deuterated compounds simultaneously and bond-selectively sensing. The 10.78 µm diameter disposable fiber probe, comprised of focusing taper as fiber probe head and time-domain walk-off eliminating fiber section with designed length, realizes wide-frequency-interval dual Stokes pulse delivering and focusing. The fiber probe enables quantitative concentration determination with resolution down to 11 mM. The chemical vibration modes of LW region C–D bonds and HW region C–H bonds of the mixture samples of organic compounds and their deuterated counterparts in a simulated cell are simultaneously excited and characterized. The CARS disposable fiber probe introduces a promising handle for in vivo biochemical detection based on isotopic labeling sensing.
Funder
National Key Scientific Instrument and Equipment Development Projects of China
National Natural Science Foundation of China
The open project of Key Laboratory of Opto-electronics Information Technology (Tianjin University), Ministry of Education
The open project of Key Laboratory of Micro Opto-electro Mechanical System Technology (Tianjin University), Ministry of Education
Tianjin Talent Development Special Plan for High Level Innovation and Entrepreneurship Team
Subject
Atomic and Molecular Physics, and Optics