Author:
Zoref Noam,Opatovski Nadav,Nehme Elias,Avrashami Maytal,Barzilai Ilana,Orange Reut Kedem,Ferdman Boris,Alalouf Onit,Azagury Dana Meron,Shamay Yosi,Shechtman Yoav
Abstract
We combine point spread function engineering with high-throughput microscopy for three-dimensional (3D) imaging. This includes novel hardware configurations and corresponding likelihood-based and neural-net-based image analysis, which is demonstrated on volumetric characterization of cancer cell spheroids.