Affiliation:
1. Imperial College London
Abstract
Structured illumination can reject out-of-focus signal from a sample,
enabling high-speed and high-contrast imaging over large areas with
widefield detection optics. However, this optical sectioning technique
is currently limited by image reconstruction artefacts and poor
performance at low signal-to-noise ratios. We combine multicolour
interferometric pattern generation with machine learning to achieve
high-contrast, real-time reconstruction of image data that is robust
to background noise and sample motion. We validate the method
in silico and demonstrate imaging of diverse
specimens, from fixed and live biological samples to synthetic
biosystems, reconstructing data live at 11 Hz across a 44 ×
44μm2 field of view, and
demonstrate image acquisition speeds exceeding 154 Hz.
Funder
Michael J. Fox Foundation for
Parkinson's Research
Alzheimer’s Research
UK
Royal Society
H2020 European Research
Council
Biotechnology and Biological Sciences
Research Council
Infinitus Ltd.
Medical Research Council
Wellcome Trust
Engineering and Physical Sciences
Research Council
Subject
Atomic and Molecular Physics, and Optics,Biotechnology