Comparative Histological Study of The Role of Platelet-Rich Plasma And Fetal Bovine Serum Supplemented Stem Cell in Treatment of Sciatic Nerve Crush Injury in Albino Rat

Abstract

Background Injuries to peripheral nerves are one of the most serious obstacles that remain without solution for now. Although there are obvious advances in microsurgery, severe types of nervous system injuries are very difficult to cure without complications. Aim To evaluate and compare the effect of platelet-rich plasma-supplemented bone marrow mesenchymal stem cells (PRP-supplemented MSCs) and fetal bovine serum-supplemented bone marrow mesenchymal stem cells (FBS-supplemented MSCs) in treatment of surgically-induced sciatic nerve crush injury. Materials and methods Seventy five adult male albino rats were equally divided into five groups 15 each. Sciatic nerve specimens were obtained and examined by light microscope. Results Compared with control group, the sciatic nerve specimens of the injury group showed loosely packed nerve fibers with perivascular cellular infiltration surrounded by discontinuous perineurium. On the other hand, both FBS-supplemented MSCs group and PRP-supplemented MSCs group showed improvement in these changes. But the improvement was markedly observed in PRP-supplemented MSCs group more than FBS-supplemented MSCs. Conclusion PRP-supplemented MSCs can reduce the pathological changes induced by crush injury to the sciatic nerve of adult male albino rats more than FBS-supplemented MSCs.