Effect of Extremely Low Frequency Electromagnetic Field on MAP2 and Nestin Gene Expression of Hair Follicle Dermal Papilla Cells

Abstract

Introduction In recent years, the extremely low frequency electromagnetic field (ELF-EMF) has attracted a great deal of scientific interest. The ELF-EMF signal is able to control ion transport across ion channels and therefore induce cell differentiation. Aim The purpose of this study was to investigate the effect of ELF-EMF (50 Hz, 1 mT) on MAP2 and Nestin gene expression of dermal papilla mesenchymal cells (DPCs). Methods In order to examine the effect of chemical and electromagnetic factors on gene expression, 4 experimental groups, namely chemical (cell exposure to chemical signals), EMF (exposing cells to ELF-EMF), chemical-EMF (subjecting cells to chemical signals and ELF-EMF) and control (with no treatment) groups, were prepared, treated for 5 days, and studied. To assess the effect of extended test time on the expression of neural differentiation markers (Nestin and MAP2), an EMF group was prepared and treated for a period of 14 consecutive days. The beneficial role of EMF in inducing neural differentiation was shown by real-time PCR analysis. Results The higher expression of MAP2 after 14 days compared to that after 5 days and decrease of cell proliferation on days 5 to 20 were indicative of the positive effect of extending treatment time on neural differentiation by evaluation of gene expression in EMF group.