Polyurethanes as Supports for Human Retinal Pigment Epithelium Cell Growth

Author:

Da Silva Gisele R.1,Armando Da S. C.2,Saliba Juliana B.2,Berdugo Marianne3,Goldenberg Brigitte T.3,Naud Marie C.3,Ayres Eliane4,Oréfce Rodrigo L.4,Cohen Francine B.356

Affiliation:

1. School of Pharmacy, Federal University of São João Del Rei, Chanadour, Divinópolis - Brazil

2. School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte - Brazil

3. INSERM UMR872, Physiopathology of Ocular Diseases: Therapeutic Innovations, Institut des Cordeliers, Paris - France

4. Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, Belo Horizonte - Brazil

5. Fondation Ophtalmologique Adolphe de Rothschild, Paris - France

6. Université René Descartes, Hotel Dieu University Hospital, Paris - France

Abstract

Purpose The transplant of retinal pigment epithelium (RPE) cells on supports may well be an effective therapeutic approach to improve the visual results of patients with age-related macular degeneration. In this study, two biodegradable polyurethanes were investigated as supports for human RPE cells (ARPE-19). Methods Polyurethane aqueous dispersions based on poly(caprolactone) and/or poly(ethylene glycol) as soft segments, and isophorone diisocyanate and hydrazine as hard segments were prepared. Polyurethane films were produced by casting the dispersions and allowing them to dry at room temperature for one week. The ARPE-19 cells were seeded onto the polyurethane films and they were investigated as supports for in vitro adhesion, proliferation, and uniform distribution of differentiated ARPE-19 cells. Additionally, the in vivo ocular biocompatibility of the polyurethane films was evaluated. Results The RPE adhered to and proliferated onto the polyurethane supports, thus establishing cellPUD surface interactions. Upon confluence, the cells formed an organized monolayer, exhibited a polygonal appearance, and displayed actin filaments which ran along the upper cytoplasm. At 15 days of seeding, the occluding expression was confirmed between adjacent cells, representing the barrier functionality of epithelial cells on polymeric surfaces and the establishment of cell-cell interactions. Results from the in vivo study indicated that polyurethanes exhibited a high degree of short-term intraocular biocompatibility. Conclusions Biodegradable polyurethane films display the proper mechanical properties for an easy transscleral-driven subretinal implantation and can be considered as biocompatible supports for a functional ARPE-19 monolayer.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biomaterials,General Medicine,Medicine (miscellaneous),Bioengineering

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