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4. Leaves of Buxus sempervirens (4 kg) were extracted by MeOH-H2O-CH3COOH (10:10:1). Usual liq./liq. partitions followed by chromatographic separations over neutral alumina and silica gel afforded 1 (15 mg) as a cristalline powder, [αD], +36 (c 0.31 in CHCl3), IR (neat) 3400, 2920, 2852, 1712, 1463, 1031 cm−1.
5. Biogenesis of cyclobuxine-D and cyclovirobuxine-D in Buxus sempervirens