Protocol for generation of CRISPR-Cas9-mediated specific genomic insertion of P2A-Gal4 to reveal endogenous gene expression in Drosophila

Author:

Chen LiminORCID,Qiao BenjiangORCID,Li Hong,Zhang Pumin,Li QiaoranORCID

Publisher

Elsevier BV

Reference11 articles.

1. FA2H controls cool temperature sensing through modifying membrane sphingolipids in Drosophila;Li;Curr. Biol.,2024

2. Multiplex genome engineering using CRISPR/Cas systems;Cong;Science,2013

3. Ends-out, or replacement, gene targeting in Drosophila;Gong;Proc. Natl. Acad. Sci. USA,2003

4. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice;Kim;PLoS One,2011

5. Gene expression systems in Drosophila: a synthesis of time and space;McGuire;Trends Genet.,2004

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