Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and β-mercaptoethanol

Author:

Kumar Ashu,Tiwari Sapana,Thavaselvam Duraipandian,Sathyaseelan Kannusamy,Prakash Archana,Barua Anita,Arora Sonia,Kameswara Rao M.

Publisher

Elsevier BV

Subject

Biotechnology

Reference25 articles.

1. Production of recombinant proteins in Escherichia coli;Schumann;Genet. Mol. Biol.,2004

2. Antibody response to Brucella melitensis outer membrane antigens in naturally infected and Rev1 vaccinated sheep;Zygmunt;Vet. Microbiol.,1994

3. Enzyme-linked immunosorbent assay for differentiation of the antibody response of cattle naturally infected with Brucella abortus or vaccinated with strain 19.;Nielsen;Am. J. Vet. Res.,1989

4. Cloning of a Brucella melitensis group 3 antigen encoding Omp28, a protein recognized by the humoral immune response during human brucellosis;Lindler;Infect. Immun.,1996

5. Recombinant OMP28 antigen-based indirect ELISA for serodiagnosis of bovine brucellosis;Chaudhuri;Mol. Cell. Probes,2010

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