Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cells 18S rDNA locus-Reference-Cited by-同舟云学术

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Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cells 18S rDNA locus

Published:2024-05 Issue: Volume:217 Page:106445
ISSN:1046-5928
Container-title:Protein Expression and Purification
language:en
Short-container-title:Protein Expression and Purification

Author:

Bayat Hadi,Farahmand Faranak,Tabatabaee Sayed Hassan,Shams Forough,Mohammadian Omid,Pourmaleki Es'hagh,Rahimpour Azam^ORCID

Funder

Iran National Science Foundation

Publisher

Elsevier BV

Reference28 articles.

1. Recent advances in CHO cell line development for recombinant protein production;Tihanyi;Drug Discov. Today Technol.,2020

2. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems;Kawabe;J. Biosci. Bioeng.,2018

3. Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications;Hertel;Front. Bioeng. Biotechnol.,2022

4. Live analysis of position-effect variegation in Drosophila reveals different modes of action for HP1a and Su(var)3-9;Bughio;Proc. Natl. Acad. Sci. U. S. A.,2022

5. [Position effect variegation: role of the local chromatin context in gene expression regulation];Boldyreva;Mol Biol (Mosk).,2022

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