An alternative use of basic pGEX vectors for producing both N- and C-terminal fusion proteins for production and affinity purification of antibodies

Author:

Aatsinki Jyrki T.,Rajaniemi Hannu J.

Publisher

Elsevier BV

Subject

Biotechnology

Reference17 articles.

1. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase;Smith;Gene,1988

2. An improved procedure for the purification of protein fused with glutathione S-transferase;Grieco;Biotechniques,1992

3. Affinity purification of GST fusion proteins for immunohistochemical studies of gene expression;Mercado-Pimentel;Protein Expr. Purif.,2002

4. A T7 expression vector for producing N- and C-terminal fusion proteins with glutathione S-transferase;Sharrocks;Gene,1994

5. Recombinant peptides as immunogens: a comparison of protocols for antisera production using the pGEX system;Oettinger;Biotechniques,1992

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