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Sensing NADH conformation using phasor analysis on fluorescence spectra

Published:2017-11 Issue: Volume:186 Page:105-111
ISSN:1386-1425
Container-title:Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
language:en
Short-container-title:Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

Author:

Palo Dylan,Maltas Jeff,Risal Laxmi,Urayama Paul

Funder

Miami University's College of Arts and Science's Dean's Scholar program

Office of Research for Undergraduate's Undergraduate Summer Scholars program

National Science Foundation

Publisher

Elsevier BV

Subject

Spectroscopy,Instrumentation,Atomic and Molecular Physics, and Optics,Analytical Chemistry

Reference32 articles.

1. Emission properties of NADH: studies of fluorescence lifetimes and quantum efficiencies of NADH, AcPyADH, and simplified synthetic models;Scott;J. Am. Chem. Soc.,1970

2. Conformational dependence of intracellular NADH on metabolic state revealed by associated fluorescence anisotropy;Vishwasrao;J. Biol. Chem.,2005

3. In vivo monitoring of protein-bound and free NADH during ischemia by nonlinear spectral imaging microscopy;Palero;Biomed. Opt. Express,2011

4. Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in a live tissue;Stringari;Proc. Natl. Acad. Sci. U. S. A.,2011

5. Metabolic imaging using two-photon excited NADH intensity and fluorescence lifetime imaging;Vergen;Microsc. Microanal.,2012

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3. UV-excited autofluorescence responses from cells embedded in turbid media containing hemoglobin analyzed using spectral phasors;Label-free Biomedical Imaging and Sensing (LBIS) 2023;2023-03-16

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5. Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays;BMC Biochemistry;2019-04-08

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