1. Escherichia coli can be adapted to grow on PPi as the sole source of phosphorus; in this situation the cells are dependent upon intracellular inorganic pyrophosphatase activity to make Pi available. To avoid possible confusion from the inducible alkaline phosphatase of E. coli, which also has pyrophosphatase activity (see Chapter 17, by Reid and Wilson, this volume), all mutant isolation studies began with an E coli strain unable to synthesize this inducible protein.
2. Isolation from urine of pyrophosphate, a calcification inhibitor
3. Physiological Studies of Conditional Lethal Mutants of Bacteriophage T4D