Islet Preparation Purity Is Overestimated, and Less Pure Fractions Have Lower Post-Culture Viability Before Clinical Allotransplantation

Author:

Kitzmann J.P.,Karatzas T.,Mueller K.R.,Avgoustiniatos E.S.,Gruessner A.C.,Balamurugan A.N.,Bellin M.D.,Hering B.J.,Papas K.K.

Publisher

Elsevier BV

Subject

Transplantation,Surgery

Reference17 articles.

1. Collaborative Islet Transplant Registry. CITR Sixth Annual Report 2009. Available at: https://web.emmes.com/study/isl/reports/CITR%206th%20Annual%20Data%20Report%20120109.pdf. Accessed May 3, 2013.

2. Characterization of islet preparations;Colton,2007

3. Limitations in the use of fluorescein diacetate/propidium iodide (FDA/PI) and cell permeable nucleic acid stains for viability measurements of isolated islets of langerhans;Boyd;Curr Trends Biotechnol Pharm,2008

4. Quantitative analysis of cell composition and purity of human pancreatic islet preparations;Pisania;Lab Invest,2010

5. A novel method for the assessment of cellular composition and beta-cell viability in human islet preparations;Ichii;Am J Transplant,2005

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