1. Ivermectin, a new broad-spectrum antiparasitic agent
2. Drug resistance in nematodes of veterinary importance: a status report
3. Avermectin inhibition of larval development in Haemonchus contortus — Effects of ivermectin resistance
4. Ctenocephalides felis assays were conducted by the Centre for Entomological Research and Insecticide Technology, UNSW, Randwick, NSW, Australia.
5. In vitro single dose C. felis assay: the test compound was dissolved in acetone (0.5mL) and applied to the base of four 100mL flasks at a concentration of 1.26μg/cm2. Flasks were left to dry for 24h before flea exposure. Controls were prepared in the same manner, except that no test compound was dissolved in the acetone. Fifteen adult fleas, aged between 3 and 7 days postemergence, were then introduced into each flask. The top of the flasks was covered in Parafilm™ and small holes were made to allow gas exchange. The treatment flasks were held at 25±1°C and 75±5% humidity for 24h. Dead fleas in each flask were counted and the pooled data were converted to percentages.