Nested-quantitative PCR approach with improved sensitivity for the detection of low titer levels of Candidatus Liberibacter asiaticus in the Asian citrus psyllid, Diaphorina citri Kuwayama

Author:

Coy M.R.,Hoffmann M.,Kingdom Gibbard H.N.,Kuhns E.H.,Pelz-Stelinski K.S.,Stelinski L.L.

Funder

Citrus Research & Development Foundation, grant

Publisher

Elsevier BV

Subject

Microbiology (medical),Molecular Biology,Microbiology

Reference24 articles.

1. Development of primers and probes for genus and species specific detection of ‘Candidatus Liberibacter species’ by real-time PCR;Ananthakrishnan;Plant Dis.,2013

2. Huanglongbing: a destructive, newly-emerging, century-old disease of citrus;Bové;J. Plant Pathol.,2006

3. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction;Bustin;J. Biomol. Tech.,2004

4. Rapid detection and quantification of viable potato cyst nematodes using qPCR in combination with propidium monoazide;Christoforou;Plant Pathol,2014

5. Development of a nested quantitative real-time PCR for detecting Phytophthora cinnamomi in Persea americana rootstocks;Engelbrecht;Plant Dis.,2013

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