Automatic determination of ligand purity and apparent dissociation constant in buffer solutions and the for anion binding in physiological solutions from -macroelectrode measurements

Author:

Kay James W.,Steven Rachel J.,McGuigan John A.S.,Elder Hugh Y.

Publisher

Elsevier BV

Subject

Health Informatics,Computer Science Applications

Reference13 articles.

1. Comparison between measured and calculated ionised concentrations in Mg2+/ATP, Mg2+/EDTA and Ca2+/EGTA buffers; influence of changes in temperature, pH and pipetting errors on the ionised concentrations;McGuigan;Mag. Res.,2007

2. J.A.S. McGuigan, J.W. Kay, H.Y. Elder, Critical review of the methods used to measure the apparent dissociation constant and ligand purity in Ca2+ and Mg2+ buffer solutions, Prog. Biophys. Mol. Biol. 92 (2006) 333–370.

3. Use of Mg2+ and Ca2+ macroelectrodes to measure binding in extracellular-like physiological solutions;Günzel;Front. Biosci.,2005

4. Automatic determination of ligand purity and apparent dissociation constant in divalent cation buffer solutions and apparent dissociation constant in physiological solutions;Kay;J. Physiol. Proc.,2004

5. Calibration of Mg2+-selective macroelectrodes down to 1μmoll-1 in intracellular and Ca2+-containing extracellular solutions;Lüthi;Exp. Physiol.,1997

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