High level expression of Glomerella cingulata cutinase in dense cultures of Pichia pastoris grown under fed-batch conditions

Author:

Seman W.M.K. Wan,Bakar S.A.,Bukhari N.A.,Gaspar S.M.,Othman R.,Nathan S.,Mahadi N.M.,Jahim J.,Murad A.M.A.,Bakar F.D. Abu

Funder

Ministry of Science, Technology and Innovation (MOSTI)

National Science Fellowship (NSF)

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference56 articles.

1. Cloning and characterization of the cutinase-encoding gene and cDNA from the fungal plant pathogen Glomerella cingulata;Abu Bakar;Asia Pac. J. Mol. Biol.,2001

2. Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6, 6 fibers;Araújo;J. Biotechnol.,2007

3. Biotransformations using cutinase;Baker;ACS Symp. Ser.,2010

4. A rapid and sensitive method for the quantification of micrograms quantities of protein utilising the principle of protein-dye binding;Bradford;Anal. Biochem.,1976

5. Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme;Brierley;Ann. N.Y. Acad. Sci.,1990

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