Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning

Author:

Tiller Thomas,Meffre Eric,Yurasov Sergey,Tsuiji Makoto,Nussenzweig Michel C.,Wardemann Hedda

Publisher

Elsevier BV

Subject

Immunology,Immunology and Allergy

Reference46 articles.

1. Epstein–Barr virus susceptibility of normal human B lymphocyte populations;Aman;J. Exp. Med.,1984

2. Human monoclonal antibodies produced by primary in vitro immunization of peripheral blood lymphocytes;Borrebaeck;Proc. Natl. Acad. Sci. U. S. A.,1988

3. Analysis of the heavy chain repertoire of human peripheral B cells using single-cell polymerase chain reaction;Brezinschek;J. Immunol.,1995

4. Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(−)/IgM+B cells;Brezinschek;J. Clin. Invest.,1997

5. Contribution of receptor editing to the antibody repertoire;Casellas;Science,2001

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