Computer-assisted semi-rational design enhanced the enzymatic activity and protein stability of Proteinase K in calcium-free conditions

Author:

Duan Rongdi,Wang Shen,Li Zhetao,Zhang Wenjun,Wu Junteng,Jiang Yifei,Lin Qinting,Yuan Peixiong,Yue Xiaoyan,Yao Yunxiao,Xiao Xiaoyue,Xiao Yunjie,Wang ZefangORCID

Funder

National Natural Science Foundation of China

Publisher

Elsevier BV

Reference22 articles.

1. Proteinase K from Tritirachium album limber, Eur. J;Ebeling;Biochemist,1974

2. P.A.J.P.r. Insel, Inflammation and thrombosis in COVID-19 pathophysiology: proteinase-activated and purinergic receptors as drivers and candidate therapeutic targets;Sriram;Phys. Rev.,2021

3. The enzymatic activity of proteinase K is controlled by calcium, Eur. J;Bajorath;Biochemist,1988

4. Structure of a serine protease proteinase K from Tritirachium album limber at 0.98 Å resolution;Betzel;Biochemistry,2001

5. Three-dimensional structure of proteinase K at 0.15-nm resolution;Betzel;Eur. J. Biochem.,1988

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