Protocol improvement and multisite validation of a digital soft agar colony formation assay for tumorigenic transformed cells intermingled in cell therapy products

Author:

Bando Kiyoko,Kusakawa ShinjiORCID,Adachi Hideki,Yamamoto Mika,Iwata Miki,Kitanaka Atsushi,Ogimura Eiichiro,Osada Tomoharu,Tamura Maya,Terai Orie,Watanabe Takeshi,Yoda Tomomi,Yotsumoto Takafumi,Zaizen Kinuko,Sato YojiORCID

Funder

Japan Agency for Medical Research and Development

Publisher

Elsevier BV

Reference18 articles.

1. Cell-based therapy approaches: the hope for incurable diseases;Buzhor;Regen Med,2014

2. Tumorigenicity assessment of cell therapy products: the need for global consensus and points to consider;Sato;Cytotherapy,2019

3. Transcriptional dynamics of immortalized human mesenchymal stem cells during transformation;Takeuchi;PLoS One,2015

4. Correction: transcriptional dynamics of immortalized human mesenchymal stem cells during transformation;Takeuchi;PLoS One,2015

5. Outgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture;Wang;Cytotherapy,2005

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