AzeR, a transcriptional regulator that responds to azelaic acid in Pseudomonas nitroreducens

Author:

Bez Cristina1ORCID,Javvadi Sree Gowrinadh1ORCID,Bertani Iris1ORCID,Devescovi Giulia1ORCID,Guarnaccia Corrado1ORCID,Studholme David J.2ORCID,Geller Alexander M.3ORCID,Levy Asaf3ORCID,Venturi Vittorio1

Affiliation:

1. International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149 Trieste, Italy

2. Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK

3. Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, POB 12 Rehovot 761001, Israel

Abstract

Azelaic acid is a dicarboxylic acid that has recently been shown to play a role in plant-bacteria signalling and also occurs naturally in several cereals. Several bacteria have been reported to be able to utilize azelaic acid as a unique source of carbon and energy, including Pseudomonas nitroreducens . In this study, we utilize P. nitroreducens as a model organism to study bacterial degradation of and response to azelaic acid. We report genetic evidence of azelaic acid degradation and the identification of a transcriptional regulator that responds to azelaic acid in P. nitroreducens DSM 9128. Three mutants possessing transposons in genes of an acyl-CoA ligase, an acyl-CoA dehydrogenase and an isocitrate lyase display a deficient ability in growing in azelaic acid. Studies on transcriptional regulation of these genes resulted in the identification of an IclR family repressor that we designated as AzeR, which specifically responds to azelaic acid. A bioinformatics survey reveals that AzeR is confined to a few proteobacterial genera that are likely to be able to degrade and utilize azelaic acid as the sole source of carbon and energy.

Funder

This work received no specific grant from any funding agency

Publisher

Microbiology Society

Subject

Microbiology

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