Antioxidant enzyme activities in maize plants colonized with Piriformospora indica

Author:

Kumar Manoj1,Yadav Vikas1,Tuteja Narendra2,Johri Atul Kumar1

Affiliation:

1. School of Life Sciences, Jawaharlal Nehru University, New Meharuli Road, New Delhi 110067, India

2. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India

Abstract

The bioprotection performance ofPiriformospora indicaagainst the root parasiteFusarium verticillioideswas studied. We found that maize plants first grown withF. verticillioidesand at day 10 inoculated withP. indicashowed improvements in biomass, and root length and number as compared with plants grown withF. verticillioidesalone. To validate our finding that inoculation withP. indicasuppresses colonization byF. verticillioides, we performed PCR analyses usingP. indica- andF. verticillioides-specific primers. Our results showed that inoculation withP. indicasuppresses further colonization byF. verticillioides. We hypothesized that as the colonization byP. indicaincreases, the presence of/colonization byF. verticillioidesdecreases. In roots, catalase (CAT), glutathione reductase (GR), glutathioneS-transferase (GST) and superoxide dismutase (SOD) activities were found to be higher inF. verticillioides-colonized plants than in non-colonized plants. Increased activity of antioxidant enzymes minimizes the chances of oxidative burst (excessive production of reactive oxygen species), and thereforeF. verticillioidesmight be protected from the oxidative defence system during colonization. We also observed decreased antioxidant enzyme activities in plants first inoculated withF. verticillioidesand at day 10 inoculated withP. indicaas compared with plants inoculated withF. verticillioidesalone. These decreased antioxidant enzyme activities due to the presence ofP. indicahelp the plant to overcome the disease load ofF. verticillioides. We propose thatP. indicacan be used as a bioprotection agent against the root parasiteF. verticillioides.

Publisher

Microbiology Society

Subject

Microbiology

Reference65 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3