Extended phenotype of an mreB-like mutant in Azospirillum brasilense

Abstract

Tn5mutagenesis was used to generate anAzospirillum brasilenseSPF94 mutant. Genetic analysis of this mutant revealed that a homologue of themreBgene, which controls cell shape inBacillus subtilisandEscherichia coli, was inactivated. The cell-surface properties of the mutant were different from those of the parental strain. The mutant colonies were highly fluorescent when grown on plates containing Calcofluor White. Light and electron microscopy revealed that the mutant cells were round and had thicker capsules than the spiral parental strain. The mutants contained up to ten times more capsule protein than the parental strain, but lacked a 40 kDa protein that is abundant in the parental strain. The phenotype of the isolated mutant resembled that of the cyst-like differentiated forms ofAzospirillum, suggesting that themreBhomologue could be involved in differentiation.