Cellulomonas carbonis sp. nov., isolated from coal mine soil

Abstract

A Gram-positive, aerobic, motile, rod-shaped bacterium, designated strain T26T, was isolated from subsurface soil of Tianjin coal mine, China. Colonies were yellow–white, convex, circular, smooth and non-transparent on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T26T was closely related to members of the genus Cellulomonas and a member of the genus Actinotalea with 96.8–94.7 % and 96.7 % gene sequence similarities, respectively. The peptidoglycan type of strain T26T was A4β, containing l-ornithine–d-glutamic acid as the interpeptide bridge. The cell-wall sugars were rhamnose, galactose, xylose and inositol. The major fatty acids (>10 %) were anteiso-C15 : 0 (33.6 %), anteiso-C15 : 1 A (22.1 %), C16 : 0 (14.4 %) and C14 : 0 (12.1 %). The predominant respiratory quinone was MK-9(H4) and the genomic DNA G+C content was 74.4 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol-mannosides and phosphatidylinositol. Comparison of phenotypic and phylogenetic characteristics between strain T26T and related organisms revealed that the new isolate represented a novel species of the genus Cellulomonas , for which the name Cellulomonas carbonis sp. nov. is proposed. The type strain is T26T ( = CGMCC 1.10786T = KCTC 19824T = CCTCC AB2010450T).