Massilia namucuonensis sp. nov., isolated from a soil sample

Abstract

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain 333-1-0411T, was isolated from a soil sample collected from Namucuo, Tibet Autonomous Region, China and characterized in a taxonomic study using a polyphasic approach. The major fatty acid components of strain 333-1-0411T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0; its major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. Q-8 was the dominant ubiquinone, and the G+C content of the genomic DNA was 66.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 333-1-0411T fell within the evolutionary radiation encompassed by the genus Massilia . The 16S rRNA gene sequence similarities between strain 333-1-0411T and recognized species of the genus Massilia ranged from 95.4 % to 97.2 %, and the most closely related strains were Massilia flava Y9T (97.2 %) and Massilia albidiflava 45T (97.0 %). However, the DNA–DNA relatedness values between strain 333-1-0411T and M. flava Y9T and M. albidiflava 45T were 20.2 % and 27.2 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 333-1-0411T is considered to represent a novel species of the genus Massilia , for which the name Massilia namucuonensis sp. nov. is proposed. The type strain is 333-1-0411T ( = CGMCC 1.11014T = DSM 25159T).