Affiliation:
1. Departments of Molecular Evolution, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir Km 4, Torrejón de Ardoz, 28850 Madrid, Spain
2. Planetology and Habitability, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir Km 4, Torrejón de Ardoz, 28850 Madrid, Spain
3. Centro de Biología Molecular ‘Severo Ochoa’ (UAM-CSIC), Cantoblanco, 28049 Madrid, Spain
Abstract
A novel actinobacterium, designated IPBSL-7T, was isolated from a drilling core 297 m deep obtained from the Iberian Pyrite Belt. The strain was isolated anaerobically using nitrate as the electron acceptor. 16S rRNA gene sequence analysis revealed that it was related to
Tessaracoccus flavescens
SST-39T (95.7 % similarity),
Tessaracoccus bendigoensis
Ben 106T (95.7 %),
Tessaracoccus lubricantis
KSS-17SeT (95.6 %) and
Tessaracoccus oleiagri
SL014B-20A1T (95.0 %), while its similarity to any other member of the family
Propionibacteriaceae
was less than 94 %. Cells were non-motile, non-spore-forming, Gram-positive, oval to rod-shaped, and often appeared in pairs or small groups. The strain was facultatively anaerobic, oxidase-negative, catalase-positive and capable of reducing nitrate. Colonies were circular, convex, smooth and colourless. The organism could grow at between 15 and 40 °C, with an optimal growth at 37 °C. The pH range for growth was from pH 6 to 9, with pH 8 being the optimal value. Strain IPBSL-7T had peptidoglycan type A3-γ′, with ll-diaminopimelic acid as the diagnostic diamino-acid and glycine at position 1 of the peptide subunit. The dominant menaquinone was MK-9(H4) (93.8 %). The major cellular fatty acid was anteiso-C15 : 0 (55.0 %). The DNA G+C content was 70.3 mol%. On the basis of phenotypic and phylogenetic results, strain IPBSL-7T can be differentiated from previously described species of the genus
Tessaracoccus
and, therefore, represents a novel species, for which the name Tessaracoccus
lapidicaptus sp. nov. is proposed. The type strain is IPBSL-7T ( = CECT 8385T = DSM 27266T).
Funder
Spanish Consejo Superior de Investigaciones Científicas
Spanish Ministerio de Economía y Competitividad
European Research Council
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
23 articles.
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