Detection of rhabdovirus viral RNA in oropharyngeal swabs and ectoparasites of Spanish bats

Author:

Aznar-Lopez Carolina12,Vazquez-Moron Sonia12,Marston Denise A.3,Juste Javier4,Ibáñez Carlos4,Berciano Jose Miguel12,Salsamendi Egoitz5,Aihartza Joxerra5,Banyard Ashley C.3,McElhinney Lorraine36,Fooks Anthony R.36,Echevarria Juan12

Affiliation:

1. Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid 28220, Spain

2. Centro de Investigación Biomédica de Epidemiología y Salud Pública, CIBERESP, Spain

3. Wildlife Zoonoses and Vector-borne Diseases Research Group (OIE Reference Laboratory/WHO Collaborating Centre), Animal Health and Veterinary Laboratories Agency (AHVLA, Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK

4. Estación Biológica de Doñana, CSIC, Seville 41092, Andalusia, Spain

5. Department of Zoology and Animal Cell Biology, University of the Basque Country (UPV/EHU), Leioa 48940, The Basque Country, Spain

6. University of Liverpool, National Consortium for Zoonosis Research, Leahurst, Neston, South Wirral CH64 7TE, UK

Abstract

Rhabdoviruses infect a variety of hosts, including mammals, birds, reptiles, fish, insects and plants. As bats are the natural host for most members of the genus Lyssavirus, the specificity of the amplification methods used for active surveillance is usually restricted to lyssaviruses. However, the presence of other rhabdoviruses in bats has also been reported. In order to broaden the scope of such methods, a new RT-PCR, able to detect a diverse range of rhabdoviruses, was designed. The method detected 81 of 86 different rhabdoviruses. In total, 1488 oropharyngeal bat swabs and 38 nycteribiid samples were analysed, and 17 unique rhabdovirus-related sequences were detected. Phylogenetic analysis suggested that those sequences detected in bats did not constitute a monophyletic group, even when originating from the same bat species. However, all of the sequences detected in nycteribiids and one sequence obtained from a bat did constitute a monophyletic group with Drosophila melanogaster sigma rhabdovirus.

Publisher

Microbiology Society

Subject

Virology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3