Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment

Abstract

A rod-shaped bacterium, designated CL-TF13T, was isolated from a tidal flat in Ganghwa, Korea. Analysis of the 16S rRNA gene sequence revealed an affiliation with the genusTenacibaculum. The sequence similarities between CL-TF13Tand type strains of members of the genusTenacibaculumwere from 94·2 to 97·4 %. Cells were motile by means of gliding. Strain CL-TF13Tgrew on solid medium as pale-yellow colonies with an irregular spreading edge. The strain was able to grow in NaCl at a range of 3–5 %. They grew within a temperature range of 5–40 °C and at pH range of 6–10. The major fatty acids were summed feature 3 (C16 : 1ω7cand/or iso-C15 : 02-OH, 19·6 %), iso-C15 : 0(18·8 %) and iso-C17 : 03-OH (13·6 %). Fatty acids such as C18 : 3ω6c(6,9,12) (1·5 %) and summed feature 4 (iso I- and/or anteiso B-C17 : 1, 1·3 %) were uniquely found in minor quantities in CL-TF13TamongTenacibaculumspecies. The DNA G+C content was 30 mol%. According to physiological data, fatty-acid composition and 16S rRNA gene sequence, CL-TF13Tcould be assigned to the genusTenacibaculumbut distinguished from the recognized species of the genus. Therefore, strain CL-TF13T(=KCCM 42115T=JCM 13039T) represents a novel species, for which the nameTenacibaculum litoreumsp. nov. is proposed.