Three putative oxylipin biosynthetic genes integrate sexual and asexual development in Aspergillus nidulans

Abstract

Oxylipins called psi factors have been shown to alter the ratio of asexual to sexual sporulation in the filamentous fungusAspergillus nidulans. Analysis of theA. nidulansgenome has led to the identification of three fatty acid oxygenases (PpoA, PpoB and PpoC) predicted to produce psi factors. Here, it is reported that deletion ofppoB(ΔppoB) reduced production of the oleic-acid-derived oxylipin psiBβand increased the ratio of asexual to sexual spore development. Generation of the triple mutant ΔppoAΔppoBΔppoCresulted in a strain deficient in producing oleic- and linoleic-acid-derived 8′-hydroxy psi factor and caused increased and mis-scheduled activation of sexual development. Changes in asexual to sexual spore development were positively correlated to alterations in the expression ofbrlAandveA, respectively. PpoB and/or its products antagonistically mediate the expression levels ofppoAandppoC, thus revealing regulatory feedback loops among these three genes. Phylogenetic analyses showed thatppogenes are present in both saprophytic and pathogenic Ascomycetes and Basidiomycetes, suggesting a conserved role for Ppo enzymes in the life cycle of fungi.