Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

Author:

Banat Ibrahim M.1,Marchant Roger1,Rahman Thahira J.1

Affiliation:

1. School of Biomedical Sciences, University of Ulster, Coleraine, County Londonderry BT52 1SA, Northern Ireland, UK

Abstract

Several aerobic, motile, rod-shaped, thermophilic, spore-formingGeobacillusbacteria predominantly giving a Gram-positive staining reaction were isolated from a cool soil environment in Northern Ireland and taxonomically investigated. Two isolates, F10 and TfT, showed low 16S rRNA gene sequence similarity to recognized members of the genusGeobacillus. Phylogenetic tree investigation using neighbour-joining, maximum-likelihood and parsimony methods indicated that strains F10 and TfTrepresent a single novel species, for which the nameGeobacillus debilissp. nov. is proposed, with type strain TfT(=DSM 16016T=NCIMB 13995T) and which belongs to a subgroup of the genusGeobacilluscomprisingGeobacillus toebiiandGeobacillus caldoxylosilyticus. However,G. debilisshowed closest affinities toBacillus pallidus, which we propose should becomeGeobacillus palliduscomb. nov.

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

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