Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori-Reference-Cited by-同舟云学术

Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori

Published:2013-05-01 Issue:5 Volume:62 Page:761-765
ISSN:0022-2615
Container-title:Journal of Medical Microbiology
language:en
Short-container-title:

Author:

Osaki Takako¹,Okuda Masumi²,Ueda Junko³,Konno Mutsuko⁴,Yonezawa Hideo¹,Hojo Fuhito¹,Yagyu Kiyoko³,Lin Yingsong³,Fukuda Yoshihiro²,Kikuchi Shogo³,Kamiya Shigeru¹

Affiliation:

1. Department of Infectious Diseases, Kyorin University School of Medicine, Tokyo, Japan

2. Department of General Medicine and Community Health Science, Hyogo College of Medicine, Hyogo, Japan

3. Department of Public Health, Aichi Medical University School of Medicine, Aichi, Japan

4. Department of Pediatrics, Sapporo Kosei General Hospital, Sapporo, Japan

Abstract

This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori to analyse intra-familial transmission. Faecal DNA was extracted and amplified by nested PCR. The products were analysed by direct sequencing and the allele type was determined using an MLST website. Mother-to-child transmission was suspected in at least two of three families, and father-to-child transmission was suspected in one family.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

Reference21 articles.

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