Chryseobacterium solani sp. nov., isolated from field-grown eggplant rhizosphere soil

Abstract

Strain THG-EP9T, a Gram-stain-negative, aerobic, motile, rod-shaped bacterium was isolated from field-grown eggplant (Solanum melongena) rhizosphere soil collected in Pyeongtaek, Gyeonggi–do, Republic of Korea. Based on 16S rRNA gene sequence comparisons, strain THG-EP9T had closest similarity with Chryseobacterium ginsenosidimutans THG 15T (97.3  % 16S rRNA gene sequence similarity), Chryseobacterium soldanellicola PSD1-4T (97.2 %), Chryseobacterium zeae JM-1085T (97.2 %) and Chryseobacterium indoltheticum LMG 4025T (96.8 %). DNA–DNA hybridization showed 5.7 % and 9.1 % DNA reassociation with Chryseobacterium ginsenosidimutans KACC 14527T and Chryseobacterium soldanellicola KCTC 12382T, respectively. Chemotaxonomic data revealed that strain THG-EP9T possesses menaquinone–6 as the only respiratory quinone and iso-C15 : 0 (29.0 %), C16 : 0 (12.5 %) and iso-C17 : 0 3-OH (11.9 %) as the major fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified glycolipids, six unidentified aminolipids and two unidentified polar lipids. The DNA G+C content was 35.3 mol%. These data corroborated the affiliation of strain THG–EP9T to the genus Chryseobacterium. Thus, the isolate represents a novel species of this genus, for which the name Chryseobacterium solani sp. nov. is proposed, with THG-EP9T ( = KACC 17652T = JCM 19456T) as the type strain.