Thermodesulfatator atlanticus sp. nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent

Author:

Alain Karine1,Postec Anne1,Grinsard Elodie1,Lesongeur Françoise1,Prieur Daniel1,Godfroy Anne1

Affiliation:

1. UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes, IUEM, Technopôle Brest-Iroise, F-29280 Plouzané, France

Abstract

A novel, strictly anaerobic, thermophilic, sulfate-reducing bacterium, designated strain AT1325T, was isolated from a deep-sea hydrothermal vent at the Rainbow site on the Mid-Atlantic Ridge. This strain was subjected to a polyphasic taxonomic analysis. Cells were Gram-negative motile rods (approximately 2.4×0.6 μm) with a single polar flagellum. Strain AT1325T grew at 55–75 °C (optimum, 65–70 °C), at pH 5.5–8.0 (optimum, 6.5–7.5) and in the presence of 1.5–4.5 % (w/v) NaCl (optimum, 2.5 %). Cells grew chemolithoautotrophically with H2 as an energy source and as an electron acceptor. Alternatively, the novel isolate was able to use methylamine, peptone or yeast extract as carbon sources. The dominant fatty acids (>5 % of the total) were C16 : 0, C18 : 1 ω7c, C18 : 0 and C19 : 0 cyclo ω8c. The G+C content of the genomic DNA of strain AT1325T was 45.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain AT1325T within the family Thermodesulfobacteriaceae, in the bacterial domain. Comparative 16S rRNA gene sequence analysis indicated that strain AT1325T belonged to the genus Thermodesulfatator, sharing 97.8 % similarity with the type strain of Thermodesulfatator indicus, the unique representative species of this genus. On the basis of the data presented, it is suggested that strain AT1325T represents a novel species of the genus Thermodesulfatator, for which the name Thermodesulfatator atlanticus sp. nov. is proposed. The type strain is AT1325T (=DSM 21156T=JCM 15391T).

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

Reference24 articles.

1. Caminibacter hydrogeniphilus gen. nov., sp. nov. a novel thermophilic, hydrogen-oxidizing bacterium isolated from an East Pacific Rise hydrothermal vent;Alain;Int J Syst Evol Microbiol,2002

2. Basic local alignment search tool;Altschul;J Mol Biol,1990

3. A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria;Cord-Ruwisch;J Microbiol Methods,1985

4. Evolutionary trees from DNA sequences: a maximum likelihood approach;Felsenstein;J Mol Evol,1981

5. Confidence limits on phylogenies: an approach using the bootstrap;Felsenstein;Evolution,1985

Cited by 42 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3